Journal: Cell Reports Methods

Article Title: Accelerated production of human epithelial organoids in a miniaturized spinning bioreactor

doi: 10.1016/j.crmeth.2024.100903

Figure Lengend Snippet:

Article Snippet: CTS TrypLE Express Enzyme no phenol red , Fisher Scientific , Catalog # 12604013.

Techniques: Recombinant, Software

Journal: eLife

Article Title: Evaluation of Gremlin-1 as a therapeutic target in metabolic dysfunction-associated steatohepatitis

doi: 10.7554/eLife.95185

Figure Lengend Snippet: ( A ) Representative RNAscope in situ hybridisation (ISH) images for co-staining of GREM1 (red) and THY1 (green) in normal human liver and MASH fibrosis. Scale bar represents 100 μM. ( B ) Quantification of ISH staining areas across different stages of liver fibrosis. Significance was assessed by two-sided Jonckheere-Terpstra test (***p=1.3 × 10 –09 ). ( C ) Quantification of human GREM1 qPCR across chronic liver diseases of different aetiology. Data are given as mean -ΔΔCt ± SD, relative to donor liver and normalised to the expression of SRSF4, HPRT1, and ERCC3. Significance was assessed by multiple two-sided Welch’s t-test against donor control, followed by Bonferroni-Holm adjustment (*p<0.05, **p=0.004). ( D ) Representative histological images of RNAscope in situ hybridisation (ISH) for co-staining of GREM1 (red) and THY1 or COL3A1 (green) in MASH fibrosis. Representative double positive cells are indicated by arrows. Scale bar represents 50 μM. ( E ) Quantification of qPCR for GREM1 mRNA in major primary human non-parenchymal cell types. HSEC – human sinusoidal endothelial cells, BEC – biliary epithelial cells, HHSC – human hepatic stellate cells, MF – myofibroblasts. ( F ) Representative RNAscope ISH images for GREM1 (red) in rats fed a standard chow or choline-deficient, L-amino acid defined high-fat diet (CDAA-HFD) for 12 weeks. Scale bar represents 50 μM. Figure 1—source data 1. Excel spreadsheet containing data displayed in panels A, C, and E.

Article Snippet: Myofibroblasts were grown on uncovered polystyrene culture plates in 16% FCS in DMEM supplemented with 1% penicillin-streptomycin-L-glutamine and subcultured at a 1:3 ratio using Gibco TrypLE Express Enzyme (12605010, Fisher Scientific) for cell dissociation.

Techniques: RNAscope, In Situ, Hybridization, Staining, Expressing, Control

Journal: eLife

Article Title: Evaluation of Gremlin-1 as a therapeutic target in metabolic dysfunction-associated steatohepatitis

doi: 10.7554/eLife.95185

Figure Lengend Snippet: ( A ) Fibrogenic marker genes in primary human hepatic stellate cells treated with anti-Gremlin-1 (aG1) or isotype control antibodies (iso-Ab). ( B ) Fibrogenic marker genes in primary human hepatic myofibroblasts treated with anti-Gremlin-1 or isotype control antibodies. ( C ) Fibrogenic gene expression in lentivirally transduced human hepatic stellate cells (HHSC). ( D ) Fibrogenic gene expression in lentivirally transduced LX-2. ( E ) Bone morphogenetic protein (BMP) signalling-related gene expression in lentivirally transduced LX-2. ( A–B ) Data are presented as individual data points and mean for -ΔΔCt relative to untreated control and normalised to the expression of SRSF4. *p<0.05 in one-way ANOVA and post hoc paired t-tests for pre-defined comparisons with Bonferroni-Holm adjustment. ( C–E ) Data are given as mean ± SEM of -ΔΔCt relative to GFP and vehicle control and normalised to the expression of SRSF4. *p<0.05 in GREM1 vs GFP-control, #p<0.05 in TGFβ1 vs vehicle control in repeated measures two-way ANOVA and post hoc paired t-test for pre-selected comparisons and Bonferroni-Holm adjustment. Figure 4—source data 1. Excel spreadsheet containing data displayed in panels A–E.

Article Snippet: Myofibroblasts were grown on uncovered polystyrene culture plates in 16% FCS in DMEM supplemented with 1% penicillin-streptomycin-L-glutamine and subcultured at a 1:3 ratio using Gibco TrypLE Express Enzyme (12605010, Fisher Scientific) for cell dissociation.

Techniques: Marker, Control, Gene Expression, Expressing